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Update(MM/DD/YYYY):08/28/2019

Confirmation of the Biodegradability of Super Growth Single-Walled Carbon Nanotubes

– A technique for measurement of carbon nanotube biodegradation in macrophages –


Researchers: Minfang Zhang, Senior Researcher and Toshiya Okazaki, Leader (also Deputy Director of the center), the CNT Characterization Team, the CNT-Application Research Center


Summary

Using an AIST original technique for quantifying the intracellular amount of carbon nanotube (CNT) that uses the near-infrared absorption measurement, the researchers measured the amount change of super-growth single-walled CNT (SG-CNTs) in macrophages over time, and clarified that SG-CNT is biodegradable, in collaboration with Zeon Corporation.

Figure
Conceptual diagram of CNT biodegradation in a macrophage


Social Background of Research

With the increase of CNT industrialization, the concern regarding the harmful effects on the public health has attracted much attention due to that CNTs are possible to be exposed to the environment, absorbed by animal and human bodies, and accumulated in immune tissues. Clarification of the biodegradation properties of CNTs is important for understanding their long-term safety. However, most of the studies on the biodegradation of CNTs are performed qualitatively, and the details are still not clear.

Details of Research

By utilizing the characteristic of CNTs that absorbs near-infrared light, the researchers have developed a technique to measure the amount change of CNT inside of macrophage cells over time. After SG-CNTs were administered and internalized into macrophage cells, the cells were lysed and the amount of SG-CNTs in the lysate was measured based on the near-infrared light absorbance. The results showed that the intracellular amount of SG-CNTs decreased over time. In addition, the amount of reactive oxygen species (ROS) generated by macrophages after SG-CNT uptake was measured. It was found that the decrease tendency of ROS coincides with that of the amount decrease of SG-CNTs inside of cells, suggesting that the biodegradation of SG-CNT in macrophages is caused by ROS.

Future Plans

The researchers will clarify the relationship between biodegradability of CNTs and their physicochemical properties such as size and surface modifications, and develop methods to enable prediction and control of biodegradability of CNTs.





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