Vol.8 No.2 2015
44/58

Research paper : Detection of influenza viruses with the waveguide mode sensor (K. AWAZU et al.)−101−Synthesiology - English edition Vol.8 No.2 (2015) through the collimator lens and optical fiber and reached the spectrometer. The two goniometers for sweeping while synchronizing became unnecessary in this optical system, and size reduction (30 cm × 15 cm × 20 cm) as shown in Fig. 6(b) was attained. This device was realized as a product by a company to which the technology was transferred. This device was equipped with a PC, Bluetooth communication, and a battery. We worked on further size reduction of the optical system, and achieved a 7 cm × 5 cm × 15 cm device as shown in (c).3.2 Identification of influenza subtypesThe identification of influenza subtypes was attempted using the waveguide mode sensor.[20] The polyclonal antibodies were obtained by immunizing healthy rabbits with H3N2 A/Udorn/307/197. These polyclonal antibodies were fixed onto the detection plate surface. After decomposing the four types of H3N2 influenza virus with surfactant Triton X-100, these were reacted with the polyclonal antibodies fixed on the detection plate surface. Then, it was highly sensitized using a label in which the polyclonal antibodies were fixed around the gold nanoparticles. The virus used in measurement was 1 g. Figure 7 shows the results, and the dashed line shows the reaction with the virus alone, while the solid line shows the reaction of antibodies highly sensitized with gold nanoparticles. The virus types were: (a) A/Shandong/9/1993, (b) A/Brisbane/10/2007, (c) A/Panama/2007/1999, and (d) A/Wisconsin/67/2005. Changes in spectrum reflectivity were confirmed in all types, and although there were differences in reactivity, it was found that H3N2 bonded with the antibodies of the same H3N2. The error was within 20 % for reflectivity changes.Next, the anti-H3 antibodies were fixed on the surface and Fig. 6 Process of size reduction of the wavelength mode sensor(a) Device number one. The optical system was set on a two-stage plate of 1 m × 50 cm. (b) A small device (30 cm × 15 cm × 20 cm) was commercialized as a product from a company to which the technology was transferred. This device was equipped with a PC, Bluetooth communication, and a battery. Further size reduction was conducted, and as shown in (c), device size of 7 cm × 5 cm × 15 cm was achieved.[19]Fig. 7 Results of detection for various types of H3N2 virus using the detection plate to which the H3N2 antibodies were fixedThe subtypes were (a) A/Shandong/9/1993, (b) A/Brisbane/10/2007, (c) A/Panama/2007/1999, and (d) A/Wisconsin/67/2005. Reflectivity (%) Reflectivity (%) Reflectivity (%) Reflectivity (%) Wavelength (nm) Wavelength (nm) Wavelength (nm) Wavelength (nm) (c) A/Panama/2007/1999 (d) A/Wisconsin/67/2005 (b) A/Brisbane/10/2007 (a) A/Shandong/9/1993Intact virus Intact virus 58056054052050048050607080901005805605405205004805060708090100Intact virus 5805605405205004805060708090100Intact virus 5805605405205004805060708090100(a)(c)(b)

元のページ 

page 44

※このページを正しく表示するにはFlashPlayer10.2以上が必要です