Vol.4 No.1 2011

10/78

Research paper : Development of novel chemical reagents for reliable genetic analyses (Y. Komatsu et al.)−7−Synthesiology - English edition Vol.4 No.1 (2011) good patents and supports such endeavors.6 Issues for the futureIn the development of the two types of reagents, the initial goal was achieved by conducting research to improve the performance of the existing reagents. However, we also realized that the existing technologies were deeply integrated into many systems, and time is needed for replacing them. Therefore, for the next development, we wanted to attempt an approach of proposing a totally new idea without concern for the immediate demand, and we recently developed a reagent that has unique properties (Fig. 7; Development 3). Although the details of this reagent are unpublished, we have filed for its patent. This third reagent has properties unseen before, but does not necessarily fulfill the users’ specific demand. Therefore, unlike the earlier reagent developments, we have no image of the final product at this moment. For this third reagent, we must create the demand on our own, or present this technology to the world through papers and hope others will come up with ideas for its use.Although there are numerous discussions on potential and demand (or “seeds and needs”), we have no idea which approach is the best from our experience in development. However, since unforeseen events do occur, there is the danger that if the researchers concentrate too much on potential and demand, they may become stalled and be unable to accomplish much. Both potential and demand are necessary to realize a product, and it is necessary to maintain the balance of the two in the process of research and revise them if necessary. We also believe that rather than setting licensing of the product as the goal, a venture spirit is needed for the true realization of a product.AcknowledgementsWe express our gratitude to the contract employees; people of the DNA Chip Research Inc., Hitachi Software Engineering Co., Ltd., Sigma-Aldrich Japan Inc., Toray Industries, Inc., and NGK Insulators, Ltd.; and the people of the former Research Institute of Genome-based Biofactory.J. Shendure and J. Hanlee: Next-generation DNA sequencing, Nat. Biotechnol., 26, 1135-1145 (2008).H. Sara, O. Kallioniemi and M. Nees: A decade of cancer gene profiling: from molecular portraits to molecular function, Methods Mol. Biol., 576, 61-87 (2010).N. Kojima, M. Sugino, A. Mikami, K. Nonaka, Y. Fujinawa, I. Muto, K. Matsubara, E. Ohtsuka and Y. Komatsu: Enhanced reactivity of amino-modified oligonucleotides by insertion of aromatic residue, Bioorg. Med. Chem. Lett., 16, 5118-5121 (2006).Y. Komatsu, N. Kojima, M. Sugino, A. Mikami, K. Nonaka, Y. Fujinawa, T. Sugimoto, K. Sato, K. Matsubara and E. [1][2][3][4]References[5][6][7]Ohtsuka: Novel amino linkers enabling efficient labeling and convenient purification of amino-modified oligonucleotides, Bioorg. Med. Chem., 16, 941-949 (2008).N. Kojima, T. Takebayashi, A. Mikami, E. Ohtsuka and Y. Komatsu: Efficient synthesis of oligonucleotide conjugates on solid-support using an (aminoethoxycarbonyl) aminohexyl group for 5’-terminal modification, Bioorg. Med. Chem. Lett., 19, 2144-2147 (2009).K. Cole, V. Truong, D. Barone and G. McGall: Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes. Nucleic Acids Res., 32, e86 (2004).N. Kojima, T. Takebayashi, A. Mikami, E. Ohtsuka and Y. Komatsu: Construction of highly reactive probes for abasic site detection by introduction of an aromatic and a guanidine residue into an aminooxy group, J. Am. Chem. Soc., 131, 13208-13209 (2009).AuthorsYasuo KomatsuCompleted the doctorate course at the Faculty of Pharmaceutical Sciences, Hokkaido University in 1995. Doctor (Pharmacology). Assistant at the Faculty of Pharmaceutical Sciences, Hokkaido University in 1995. Manager of DNA Chip Research Inc. in 2000. Senior researcher of the Institute for Biological Resources and Functions, AIST in 2003. Research Institute of Genome-based Biofactory in 2005. Leader of the Biomolecular Engineering Research Group, Bioproduction Research Institute, AIST in 2010. In this paper, was in charge of proposal of the theme, integration, and synthesis and activity evaluation of the nucleic acid.Naoshi KojimaCompleted the doctorate course at the Faculty of Pharmaceutical Sciences, Hokkaido University in 1998. Doctor (Pharmacology). Joined the Hokkaido National Industrial Research Institute, Agency of Industrial Science and Technology in 2001. Researcher of Institute for Biological Resources and Functions, AIST in April 2001. Senior researcher of the Biomolecular Engineering Research Group, Bioproduction Research Institute, AIST in 2010. In this paper, was in charge of the chemical synthesis of monomers.Discussions with Reviewers1 “2 Scenario to realize the goal”Comment (Yoshifumi Jigami, Research Center for Medical Glycoscience, AIST)In this section, the explanation of Fig. 1 is difficult to understand. It may be better if you specifically explain each “elemental technology” of the genetic analysis system and their mutual relationship, as well as the relationship with “labeling” and “DNA synthesis”. Also, “conventional technology”, “first-generation type (ssN-linker)” and “second-generation type (ssH-linker)” of Fig. 3 should be compared in a table that shows their properties, advantages and disadvantages. You should explain

※このページを正しく表示するにはFlashPlayer9以上が必要です