Vol.3 No.2 2010

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Research paper : Development of an accurate and cost-effective quantitative detection method for specific gene sequences (N. Noda)−172−Synthesiology - English edition Vol.3 No.2 (2010) [10][11][12][13]quenching probe system: flexible, specific, and cost-effective real-time polymerase chain reaction method, Anal. Chem., 81, 5678-5685 (2009).H. Tani, T. Kanagawa, S. Kurata, T. Teramura, K. Nakamura, S. Tsuneda and N. Noda: Quantitative method for specific nucleic acid sequences using competitive polymerase chain reaction with an alternately binding probe, Anal. Chem., 79, 974-979 (2007).S. K. Singh, P. Nielsen, A. A. Koshkina and J. Wengel: LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition, Chem. Commun., 4, 455-456 (1998).H. Tani, T. Teramura, K. Adachi, S. Tsuneda, S. Kurata, K. Nakamura, T. Kanagawa and N. Noda: Technique for quantitative detection of specific DNA sequences using alternately binding quenching probe competitive assay combined with loop-mediated isothermal amplification, Anal. Chem., 79, 5608-5613 (2007).Nikkei Biotech (ed.): Nikkei Baio Nenkan 2010 (Nikkei Biotechnology Almanac), Nikkei BP (2009) (in Japanese).AuthorNaohiro NodaCompleted the doctoral program at the Department of Applied Chemistry, Graduate School of Science and Engineering, Waseda University in 2002. Doctoral candidate of the Research Fellowship for Young Scientist, Japan Society for the Promotion of Science from January 2000 to March 2002. Doctor (Engineering). Became Research Fellow, AIST in 2002. Researcher of the Biological Resource Information Core Research Group, Institute for Biological Resources and Functions (BRF), AIST in 2005. Researcher of Bio Measure Research Group, BRF, AIST in 2006. Researcher of Bio Measure Research Group, Biomedical Research Institute, AIST in April 2010. Studies the development and assessment of the quantification technology for nucleic acids (DNA, RNA) and the development of the high throughput activation assay of the proteins that interact with the nucleic acid.Discussions with Reviewer 1 Specific assumed client and scenario developmentComments (Yoshifumi Jigami, Evaluation Division (current affiliation: Research Center for Medical Glycoscience), AIST)I think you need to analyze the issues and problems that must be overcome to make this business successful. For example, you should consider the attributes of those whom you specifically assume to be your clients that can take advantage of “the low cost and quick delivery”, and then present the scenario to develop the business.Answer (Naohiro Noda)The assumed clients that can take advantage of “the low cost and quick delivery” include the environment companies that must monitor diverse environmental microbes in bioremediation, and the genetic testing companies that must analyze innumerable gene types. We used the example of the environment companies for monitoring the environmental microbes because their market is expected to expand in the future, and revised the manuscript accordingly.2 Issues and points for the diffusionComments (Yoshifumi Jigami)The sales plan for the inexpensive fluorescence measurement device (less than 1 million yen) with accompanying reagent kit is discussed, and its diffusion as a tool for the quantification and analysis of genes at low cost in the developing countries and others is suggested as a probable business development. While this is a very interesting suggestion, you did not give the issues and points that must be overcome to realize this. Answer (Naohiro Noda)As the issues and problems that must be overcome to realize the diffusion to developing countries, downsizing and energy saving of the developed technology are necessary. To achieve these, I think the development of the biochip that combines the micro total analysis system (-TAS), which is advancing dramatically recently, is important in the future scenario. This point was described in the revised manuscript.3 Value and social impact on the marketComments (Yoshifumi Jigami)Figure 9 shows the points for realization and the corresponding candidate products. I think it will be easier to understand if you describe the value and social impact of these products on the market.Answer (Naohiro Noda)“Value and social impact of the products on the market” was added to the product candidate in Fig. 9.4 Problems in technological development and scenario for solutionComments (Yoshifumi Jigami)As future prospects, you describe “the development of the new technology combining the universal QProbe and the ABC methods” and “the development of the on-site or mobile gene quantification device combined with the isothermal gene amplification method”. While these are important in looking at the “researcher’s dream” or the “link between the research objective and the society (social values)”, you should discuss the issues that must be overcome to achieve such technological developments, scenario to solve the issues, and the impact on the market when they are realized.Answer (Naohiro Noda)For “the development of the new technology combining the universal QProbe and the ABC methods”, it is necessary to generate ideas about joint DNA that is compatible with the ABC method by advancing the concept of current joint DNA. (The details of the idea will be omitted here since the development is in progress.) For “the development of the on-site or mobile gene quantification device combined with the isothermal gene amplification method”, selection of isothermal amplification technologies (development of new technology if necessary) and simplified nucleic acid extraction technology are needed. The issues that must be overcome for technological development and the impact on the market when they are realized are described in the revised manuscript.

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