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Research paper−43−Synthesiology - English edition Vol.3 No.1 pp.43-52 (Jul. 2010) the clinical tests to private clinical laboratories. Figure 1 shows the schematic diagram of the clinical test outsourcing based on the brain-to-brain loop model suggested by Lundberg et al[2][3]. In contrast, POCT allows the sampling and selection of tests to be done at the patient’s side, and the results can be obtained directly from the tests conducted on the spot. This enables definitive diagnosis at the patient’s first visit to a medical institution, and this will allow a swift start of the treatment, increased treatment efficiency, reduced load of repeat visits, and reduced medical expenses. This in turn will be beneficial to the patient, the medical institutions, and to society. As a benefit for the clinician, it will enable obtainment of useful, on-the-spot information for determining the treatment method, such as the presence of infections or systemic diseases and their clinical conditions in cases that may require emergency surgical intervention. Currently, POCT devices have been developed and introduced in the clinical practice for conditions that 1 IntroductionTo achieve a high quality life of good health and longevity, it is necessary to prevent various diseases by ultra early detection or predictive diagnosis, particularly of lifestyle-related diseases such as diabetes. To do so, it is necessary to monitor several biomarkers related to various diseases on a daily basis at a personal level, and to establish a network and a diagnostic system of the data obtained. To achieve these goals, we are working to construct a device to measure multiple biomarkers that can be used by patients. Ultimately, it is necessary to establish a technology to detect multiple biomarkers that are present in the humoris such as blood, in the setting of people’s daily lives and homes. To realize this, the device must: combine the processes of blood sample collection, pretreatment, separation, reaction, and detection; be compact and easy to use so it can be installed and used at home; and also enable analysis of multiple biomarkers for accurate diagnosis. The biomarker analysis conducted to observe the in vivo biological or biochemical changes of protein, glucose, and lipid in the blood is exactly the same as the clinical test conducted when a patient visits a hospital.Recently, there is an increased demand for “clinical tests at patient’s side” or point of care testing (POCT) in the field of clinical tests[1]. In current clinical testing, it is rare that the results be available on the same day as the test. Several days are required before the results become available, and this may prevent swift diagnosis and treatment. Other than the fact that the biomarker measurement itself is time consuming, large and expensive precision measurement device is necessary, and the economic burden on the medical institution is great including the cost of labor for lab technicians. Therefore, many medical institutions outsource - Development of POCT device for multi-marker analysis -Masatoshi Kataoka*, Shouki Yatsushiro, Shouhei Yamamura, Masato Tanaka and Toshihiko OoieHealth Technology Research Center, AIST (current affiliation: Health Research Institute, AIST) 2217-14 Hayashi-cho, Takamatsu 761-0395, Japan *E-mail : Original manuscript received September 1, 2009, Revisions received January 18, 2010, Accepted January 20, 2010Point of care testing (POCT), the analysis of biomarkers at the patient’s side, is a continuously expanding trend in the practice of laboratory diagnosis. Although some POCT devices for the analysis of blood glucose and/or several infectious diseases have been developed, many laboratory tests in almost all hospitals are contracted out to clinical laboratory companies. However, outsourcing of biomarker analysis is time-consuming, high in cost, and requires much blood and reagents. Consequently we are constructing a biomarker analysis system on microchips for the POCT device. In this paper, we show the core technology for the analysis of biomarkers on microchips, and describe the problems and its solutions in the application of microchips for POCT device.Biomarker analysis on microchipsKeywords : Laboratory testing, biomarker, POCT, microchip electrophoresis, microchip, multi-marker analysis[Translation from Synthesiology, Vol.3, No.1, p.16-25 (2010)]POCTDiagnosis, treatmentTest resultSample analysisStorage and transportation of sampleSample collectionIndication for testVisit to physicianFig. 1 Flow of tests currently dependent on clinical testing companies.

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