Vol.3 No.1 2010

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Research paper : A bioinformatics strategy to produce a cyclically developing project structure (M. Suwa et al.)−7−Synthesiology - English edition Vol.3 No.1 (2010) joint researches are fed back, and the current information volume is abundant. Figure 4 shows the web page (http://sevens.cbrc.jp) of the current SEVENS. The top page shows the list of the eukaryotes, and when an organism type is selected, the search page is shown. One can jump to the GPCR detailed analysis page from the chromosome map, the phylogenetic icon, or the search condition entry form. From the detailed analysis page, such information as the coordinates of selected GPCR, exon sequence, sequence resemblance search, gene expression pattern, ligand binding, G protein binding, composition of amino acid sequence, predicted transmembrane helix region, functional motif region, domain region, region predicted to be indeterminate structure (disorder region), exon-intron boundary, pseudo-gene, new gene, and 3D structure modeling can be viewed. GRIFFIN that was developed for functional prediction can be used on the web (http://griffin.cbrc.jp/). When the molecular weight of the ligand and GPCR sequence are entered, the bonding G protein is predicted. The ligand molecular weight can be set in arbitrary steps for a certain value. The step-by-step ligand molecular weight setting is useful for the prediction of bonding G protein with an orphan receptor whose ligand is unknown. 5 Jump again: future research development5.1 Understanding high-order biological phenomena Up to this point, we placed weight on the functional analysis of individual genes from a comprehensive perspective, but in the future, research to understand high-order biological phenomena based on the entire gene network is necessary. From this perspective, we started working on the system that involves the olfactory receptors that dominate the majority of the mammalian GPCR. The olfactory system induces memories and emotions through multitudinous combination of odor molecule types. Therefore, if this system can be understood systematically, it may lead to research for producing an environment that makes people feel pleasure by blending certain odor molecules. The electric activation signals from all of the several hundred olfactory receptors that respond to diverse odor molecules are integrated to form 2D patterns (odor map) on the olfactory epithelium. We would like to understand the spatiotemporal cause-and-effect relationships among odor molecules, receptors, cells, and the odor map. Specifically, we are planning to develop a program to predict the activation of all olfactory receptors against odor molecules (activation array), and apply this to all olfactory receptors of humans and mice. Fig. 4 Current SEVENS database (http://sevens.cbrc.jp). Top page: Click on organism typeSearch page: Search from chromosome mapPhylogenic tree from all sequencesSequence similarity search resultLink to Swiss-ProtExon sequenceExpression information by GENE EXPRESSPredicted transmembrane region by SOSUIPredicted disorder region by DisopredPfam domainPROSITE motif3D structure modelingEnlargement of chromosome mapKnown transcription regulatory region

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