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13AIST TODAY 2013-1life-liaison-ml@aist.go.jpFor inquiries about this article : Research Planning Office of Life Science and BlotechnologyQuantification of stemness based on cell pluripotency(A) Quantification of iPS cells measured by ourselves and their parent cells, (B) Quantification of cells that are partly pluripotent or in the differentiation stage, (C) Quantification of cancer cellsABCStemcellsSomaticcells1.00.50.0ー0.5ー1.0working together with a corporation on the development of a device that can connect the isolator to Japanese- and foreign-made cell processing units in a sterile manner so as to enhance the extensibility of the isolator.We are actively engaged in the development of cell production technology as a supporting technology for NEDO iPS cell projectWe participated in a NEDO iPS cell project in FY2009 and 2010. We used the genetic expression data of the iPS cell established in the project to investigate the genetic control network specific to iPS cells.[1] Then, we used the genetic expression data of other iPS cells established from a different parent cell and attempted to quantify stemness.Identification of pluripotent gene setsIn order to quantify stemness, we selected a gene set that serves as an indicator of cell pluripotency.First, we selected gene sets having an expression level statistically different from that of their parent cells, using iPS cell strains established from three different parent cells. Gene sets with an expression level different from that of all of their iPS parent cells were selected from ES cells. Then, a gene set that do not depend on the origin or passage of cells was selected from the gene set common to these four gene sets. According to the same selection rule, a gene set was selected from six data sets including parent cells among the open data of iPS cells originating from fibroblasts in addition to our own measured data. Lastly, we compiled all of these data and selected a final gene set. The gene set selected by this procedure is considered to be an index gene set for pluripotency (IGSP) with the effects of originating cells and strains eliminated as much as possible.Quantitative evaluation of stemnessThe expression level of iPS cells that have partial pluripotency or are considered Quantification of Stemness regenerative medicine side-by-side with clinical research on regenerative medicine, with the wish that our isolator system that can save space of production systems will disseminate to ordinary medical institutions so as to eventually help reduce the costs of cell production.Tissue Engineering Research GroupHealth Research InstituteShunsuke YUBA

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