We have developed a new technique to effectively produce D-glyceric acid from glycerol, which is in abundant supply due to the proliferation of biodiesel fuel. The total amount of glycerol produced worldwide is estimated to be around a million tons a year, and therefore, an effective use for this surplus glycerol has been sought. In our new method for producing D-glyceric acid effectively, a highly oxidative microorganism belonging to acetic acid bacteria, and a membrane that selectively permeates ions for separation and concentration of the product were used. Although D-glyceric acid and its derivatives have excellent biological functions, including an action to accelerate alcohol metabolism, they are expensive because industrial methods have not been established for their production. If they can be produced at low cost, various uses will be expected, including raw materials for chemical products such as bioplastics, pharmaceuticals for alcohol metabolism acceleration or liver disease treatment, and cosmetics.