A rapid and easy-to-use tool for stress measurement based on microchip technology and electrophoretic separation technique was developed. Cortisol and secretory immunoglobulin A (s-IgA) in saliva were measured as stress-related compounds. Advantages of microchip system over conventional immunoassay methods include short analysis times, high separation efficiency, reduced cost and disposability. The detection sensitivity was improved by the development of laser-induced fluorescence detection system, microchip devices and optimal analytical conditions. We expect our research will provide self-care products for stress diagnosis in future.