A new photo-responsive cell culture substrate has been developed. The substrate was based on photo-responsive polymer, and cell adhesion to the substrate can be modulated by irradiation of lights with specific wavelengths without affecting cell viability. This substrate enables us to control the adhesion (capturing or removing) of individual adherent cells on our demand by irradiating spot light under a microscope, and is expected to provide an innovative technique to manipulate living and adherent cells.