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AIST TODAYNo.18 Autumn 2005 [ PDF:10.2MB ]

Development of highly-integrated plastic biochips; Open the doorway to low-cost and desk-side medical diagnosis

Mitsuru Ishikawa
Health Technology Research Center
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Replica biochips for capillary array electrophoresis with 10 separation channels (50 µm width, 50 µm depth and 100 µm pitch) were successfully fabricated on a poly(methyl methacrylate) (PMMA) substrate using injection molding technique. The current fabrication method used moving mask deep X-ray lithography to fabricate an array of channels with inclined channel sidewalls. A slight inclination of channel sidewalls is highly required to ensure the release of replicated biochips from a mold. Moreover, the sealing of molded PMMA multichannel chips with a PMMA cover film was achieved using a novel bonding technique involving adhesive printing.

Figure 1
Figure 1: Schematic of a 10-channel PMMA biochip for microchannel electrophoresis. (A) Design of a biochip with 10 separation channels of 50 µm in width and 50 µm in depth. (B) -(F) close-up views of a biochip using scanning electron microscope.

Figure 2
Figure 2: Electropherograms of PCR products of a control gene (channel 1 and 2), surfactant protein (SP) genes (channel 3 to 9) which are related with human lung cancer, and a 100-bp DNA ladder (channel 10) on a 10-channel bio chip. Appearance of a control gene in channel 1 and 2 shows that the procedure is valid for all the sample preparations.

Relational Information

AIST TODAY Vol.5, No.8 (2005) p.30-31



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