Microchannel reactors have been attracting attention as a novel reaction apparatus in chemical industry. We have developed enzyme-immobilized microreactor, which can perform multistep enzyme reactions. We also developed partial surface modification method of microchannel surface for separation of aqueous and organic solution within microchannel. Using this method, we have developed efficient enzyme microreactor for optical resolution of chiral compounds. Further development of micro-bioreactor are in progress.