| Three-dimensional reconstruction from electron micrographs requires the selection of many single particle projection images; more than 10,000 are generally required to obtain 5 ~ 10 Å structural resolution. This paper presents a new automated particle recognition and pick-up procedure based on the three layer neural network. Its use for both faint and noisy electron micrographs is demonstrated. The method only requires 200 selected particles as learning data and is able to detect images of proteins as small as 200 kDa1).
1) T. Ogura & C. Sato, J. Struct. Biol., Vol. 136, 227-238 (2001).
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Schematic representation of the three-layer NN and its learning process
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| Relational Information |
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AIST Today Vol. 4, No.2 (2004) 14
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No.12 Spring 2004
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