We have developed a novel expression system in yeast, Saccharomyces cerevisiae, at low temperature. In the expression system, target proteins can be produced only by lowering a culture temperature. A production yield of a protein in the expression system was higher than that in the existing expression systems in yeast at moderate temperature. Several proteins that were expressed as insoluble forms in Escherichia coli could be produced in functional and soluble forms in our expression system. Our expression system will greatly contribute to proteomics study and large-scale production of pharmaceutical and industrial proteins.
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Effective production of EGFP3 in yeast at low temperature
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| Relational Information |
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AIST Today Vol. 3, No.12 (2003) 21
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No.11 Winter 2004
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